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T细胞疫苗接种实验性自身免疫性神经炎的实验研究
作者:吴云        梁庆成        王维治        Wu Yun        Liang Qing-cheng        Wang Wei-zhi 
单位:哈尔滨医科大学附属第二医院神经科 黑龙江 哈尔滨 150086 
关键词:T细胞疫苗接种      实验性自身免疫性神经炎 
分类号:R744.5 R392.7
出版年,卷(期):页码:2008,15(6):0-0
摘要:
目的 建立P2-特异性T细胞系,在实验性自身免疫性神经炎(EAN)大鼠进行T细胞疫苗接种(TCV)的实验研究.方法 P2多肽、IL-2和抗CD3/CD28单抗包被磁珠诱导扩增P2-特异性T细胞及非抗原特异性T细胞,将灭活T细胞接种大鼠,检测指标包括:瘫痪高峰期最大评分比较,淋巴细胞增殖试验、CD4+T/淋巴结单个核细胞及CD4+ CD25+ T/CD4+T细胞百分比测定、培养液上清IFN-γ及IL-10水平测定以及坐骨神经病理学检查.结果 P2-特异性T细胞显著扩增,2周扩增近1000倍;P2-特异性TCV预防组无大鼠发病;P2-特异性TCV治疗组大鼠病情减轻,其坐骨神经炎性细胞浸润程度显著轻于对照组(P-特异性TCV预防组和治疗组CD4+ CD25+ T/CD4+T细胞百分比及培养液上清IL-10水平均显著高于其对照组,培养液上清IFN-γ水平均显著低于其对照组(均P-特异性TCV能预防EAN发生,并可治疗已发生的EAN,其机制可能为:使CD4+ CD25+调节性T细胞/CD4+T细胞比例上调,诱导致病性CD4+Th1细胞向保护性CD4+ Th2细胞转换.
基金项目:
国家自然科学基金
作者简介:
参考文献:
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