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不同剂量牛型结核杆菌卡介苗对EAE模型的诱导
作者:刘颖        刘华        武慧丽        辛晋敏        马太花        梁丽云        马存根        LIU Ying        LIU Hua        WU Hui-li        XIN Jin-ming        MA Tai-hua        LIANG Li-yun        MA Cun-gen 
单位:刘颖 马存根 LIU Ying MA Cun-gen(山西大同大学脑科学研究所 山西 大同 037008 山西医科大学第一临床医学院神经科 山西 太原 030001)刘华 武慧丽 辛晋敏 马太花 梁丽云 LIU Hua WU Hui-li XIN Jin-ming MA Tai-hua LIANG Li-yun(山西大同大学脑科学研究所 山西 大同 037008) 
关键词:实验性自身免疫性脑脊髓炎      牛型结核杆菌疫苗      单核细胞趋化蛋白-1 
分类号:R744.5+3
出版年,卷(期):页码:2005,12(6):0-0
摘要:
目的探讨实验性自身免疫性脑脊髓炎(experimental autoimmune enccphalomyelitis,EAE)非敏感品系Wistar大鼠加用不同剂量牛型结核杆菌卡介苗(mycobacterium bovis bacillus calmette-guerin,M.bovis BCG)对EAE模型诱导的作用.方法将Wistar大鼠分为EAE1、EAE2、EAE3、EAE4共4组,然后分别用含4、8、10、12 mg M.bovis BCG/mL完全福(氏)佐剂(CFA)加豚鼠脑脊髓匀浆(GPSCH)制备不同浓度的完全抗原,分别免疫诱导Wistar大鼠EAE.观察不同剂量M.bovis BCG对诱导EAE模型发病情况、各项临床指标的影响,同时取脑和脊髓制成石蜡切片,行HE染色观察组织形态变化,行单核细胞趋化蛋白-1(MCP-1)mRNA原位杂交(ISH)计数其阳性细胞百分率.结果EAE3、EAE4组的发病率、体重减轻、临床症状评分、MCP-1 mRNA阳性细胞数显著高于EAE1、EAE2组(均P<0.05).结论M.bovis BCG浓度的高低影响Wistar大鼠EAE的发病率.
基金项目:
山西省留学回国人员科研项目
作者简介:
参考文献:
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