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帕金森病患者的外周血淋巴细胞DAT功能检测
作者:潘天虹        刘昭平        张珏        周孝达        费俭 
单位:上海第二医科大学附属仁济医院神经内科 上海 200001 
关键词:帕金森病      淋巴细胞      多巴胺转运蛋白 
分类号:R741.04
出版年,卷(期):页码:2002,9(4):0-0
摘要:
目的比较帕金森病(PD)患者与正常对照组外周血淋巴细胞摄取多巴胺(DA)量的差异.方法用密度梯度离心法提取外周血淋巴细胞,以Western blot法检测淋巴细胞上多巴胺转运蛋白(DAT蛋白),以液体闪烁记数法检测淋巴细胞摄取3H-DA的量.结果 Western blot显示在相对分子质量为66 000部位出现一与DAT蛋白大小相吻合的条带;淋巴细胞有摄取3H-DA的功能,且为时间、浓度及Na+依赖;PD患者淋巴细胞摄取3H-DA的量明显低于正常对照组(P<0.05).结论检测PD患者外周血淋巴细胞摄取3H-DA量与正常对照组之间的差异有可能成为PD诊断的一个实验室依据.
基金项目:
国家重点基础研究发展计划(973计划)
作者简介:
参考文献:
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