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趋化因子mRNA在多发性硬化中的表达
作者:周文斌        朱世津        李静        杨杰        杨欢        肖波        谢光洁 
单位:周文斌 李静 杨杰 杨欢 肖波 谢光洁(中南大学湘雅医院神经内科 )朱世津(常德市第一人民医院神经内科 ) 
关键词:多发性硬化      趋化因子      原位杂交 
分类号:R774.5+1
出版年,卷(期):页码:2001,8(4):0-0
摘要:
目的本研究观察了多发性硬化(MS)患者趋化因子mRNA在周围血及CSF中的表达. 方法采取MS患者外周血及CSF,以其他非炎性神经系统疾病(OND)及健康人(HC)为对照组,分离单个核细胞(MNC),在完全培基中与自身抗原MBP、对照抗原AChR培养,3 d后收集细胞,涂片,用地高辛标记的寡核苷酸探针进行原位杂交(ISH),检测C-C趋化因子单核细胞炎性蛋白-1α/β(MIP-1α/ β)、单核细胞趋化蛋白(MCP-1)及正常T细胞表达及分泌的调节活化因子(RANTES) m RNA的表达. 结果 MS患者外周血中MIP-1α/β、MCP-1 及RANTES mRNA阳性细胞表达,与OND组及HC组相比明显增高,差异显著;MS患者CSF中MNC的 MIP-1α及RANTES mRNA高于OND组,差异有显著性;MS急性期外周血中MBP刺激的MIP-1α mRNA与MS缓解期相比差异不明显,而急性期MBP刺激的MCP-1 mRNA 阳性细胞数多于其缓解期;MS患者急性期CSF内RANTES mRNA阳性细胞数高于缓解期. 结论 [ HT5”BS 趋化因子可能参与MS的发病,与炎性细胞进入CNS有关;RANTES及MCP-1与MS的缓解-复发有关.
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参考文献:
Steinman L Multiple sclerosis:a coordi nated immunological attack against myelin in the central nervous system 1996 Cannella B.Raine CS The adhesion molecule and cytokine profile of multiple sclerosis lesions 1995 Ransohoff RM.Glabinski A.Tani M Chemokin es in immune-mediated inflammatory of the central nervous system 1996 Poser CM.Paty DW.Scheinberg L New diagnostic criteria for multiple sclerosis:guideli ness for research protocols 1983 Deibler GE.Martensson RE.Kies MV Larg e scale preparation of myelin basic protein from central nervous tissue of sever al animal species 1972 Wang ZY.Qiao J.Link H Suppression of experimental autoimmune myasthenia gravis by oral administration of acetylcholin e receptor 1993 Ransohoff RM Chemokines in neurological disease models:correlation between chemokine expression patterns and inflammato ry pathology 1997 Biddison WE.Cruikshank.Center DM CD8+ myelin peptide-specific T cells can chemoattract CD4+ myelin peptide-specific T cells:importance of IFN-inducible protein 1 0 1998 Miyagishi R.Kikuchi S.Fukazawa T Macrophage inflammatory protein-1α in the cere brospinal fluid of patients with multiple sclerosis and other inflammatory neuro logical diseases 1995 Karpus WJ.Ransohoff RM Chemokine regulation of experimenta l autoimmune encephalomyelitis:temporal and spatial expression patterns govern disease pathogenesis 1998 Baker CM.Kolson DL Chemokines and chemokine receptors in the pathogenesis of multiple sclerosis 2000(01) Van Der Voorn P.Tekstra J.Beelen RH Expression of MCP-1 by reactive astrocytes in demyelinating multiple sclerosis lesions 1999 周文斌.HansLink 多发性硬化患者周围血单个核细胞的IL-10分泌细胞变化及意义 [期刊论文] -中华神经科杂志1998 Juebor MN.Das AM.Perretti M R egulation of macrophage inflammatory protein-1 alpha expression and function by endogenous interleukin-10 in a model of acute inflammation 1999
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